Optimization of Catalytic Reaction Conditions for Glucose Oxidase in Microcalorimeter by Response Surface Methodology

Abstract

Abstract: Response surface methodology based on a central composite design was used to optimize catalytic reaction conditions for glucose oxidase in micro-differential scanning calorimeter (Micro DSC) based on heat release. Three reaction conditions in decreasing order of their effects on heat release were enzyme dosage, pH and reaction temperature and their optimal values were 1.58 mg/mL, 4.46 and 35 ℃, respectively. Under these conditions, the observed total heat release was 39.6668 J, and the relative error was 1.11% compared with 40.1071 J of the predicted value. The fitted regression model was statistically significant.v

Key words: microcalorimetric method, glucose oxidase, response surface methodology

CLC Number:

TS201.6

Cheng-Nan ZHANG Fei Xu. Optimization of Catalytic Reaction Conditions for Glucose Oxidase in Microcalorimeter by Response Surface Methodology[J]. FOOD SCIENCE, 2012, 33(23): 166-170.

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