Screening of Cellulase-Producing Bacterium from Sorbus tianshanica and Enzymatic Characteristics

Abstract

Abstract: Fifty-three endophytic bacteria were isolated from Sorbus tianshanica Rupr. stems, leaves and fruits. Four colonies were surrounded by big transparent zones in congo red agar medium. A strain (S11) with higher cellulase activity was isolated and cultured in shake flasks for secondary screening. Maximum carboxymethyl cellulase (CMCase) activity was found to be 39.47 U/mL after 12 h culture. The optimal reaction pH for crude CMCase solution was 5.0, and it revealed high stability at pH 5.0–6.0. The optimal reaction temperature was 50 ℃, and its activity remained stable below 50 ℃ but was almost completely lost at 60–70 ℃. The CMCase activity was inhibited by all tested metal ions and compounds, and dramatically inhibited by Ca2+, SDS and EDTA.

Key words: Sorbus tianshanica Rupr., cellulase-producing bacterium, cellulase activity, enzymatic characteristics

CLC Number:

Q814.1

References

[1] 于明, 李铣. 花楸属植物化学成分药理作用及其研究进展[J]. 辽宁中医学院学报, 2004,6(5):364-366.
[2] 李迪民, 陈坚．天山花楸水提取液药理学研究[J]．中草药, 1996, 26(6):302．
[3] 李迪民, 陈坚．天山花楸醇提取物对心肌缺血的保护作用[J]．西北药学杂志, 1998, 13(2): 66．
[4] 刘艺, 翁幼武, 张琦．HPLC法测定天山花楸枝叶中槲皮素含量[J]．广西医学, 2005,27(6):839．
[5] 张芹, 刘保刚, 李鹏, 等. 天山花楸中总黄酮和槲皮素测定[J]. 石河子大学学报（自然科学版）, 2006, 24(6):709-712.
[6] 李景庄, 胡君萍, 杨建华, 等. HPLC－UV法测定天山花楸中金丝桃苷和芦丁的含量[J]. 药物分析杂志, 2009, 29 (2):310-312.
[7] 张芹, 李鹏, 唐辉, 等. 高效液相色谱法测定天山花楸中的绿原酸含量[J]. 时珍国医国药, 2008, 19(1):59-60.
[8] Miroslava Ka?ániová, Martina Fikselová. Mycological flora on tree fruits, crust, leaves and pollen Sorbus domestica L[J]. Ann Agric Environ Med. 2007, 14(2): 229-232.
[9] Izumi H, Anderson IC, Killham K, et al. Diversity of predominant endophytic bacteria in European deciduous and coniferous trees[J]. Can J Microbiol. 2008, 54(3): 173-179.
[10] Taeechowisan T, Peberdy J F, Lumyyong S．Isolation of endophytic actinomycetes from selected plants and their antifungal activity. World J Microbiol Biotech, 2003, 19:381-385.
[11] Kang DG, Sohn EJ, Lee AS, et al. Methanol extract of Sorbus commixta cortex prevents vascular inflammation in rats with a high fructose-induced metabolic syndrome[J]. Am J Chin Med. 2007, 35(2):265-277.
[12] Termentzi A, Alexiou P, Demopoulos VJ, et al. The aldose reductase inhibitory capacity of Sorbus domestica fruit extracts depends on their phenolic content and may be useful for the control of diabetic complications[J]. Pharmazie. 2008, 63(9): 693-696.
[13] 杨柳, 魏兆军, 朱武军, 等. 产纤维素酶菌株的分离、鉴定及其酶学性质研究[J]. 微生物学杂志, 2008, 28(4): 65-69.
[14] 游庆红, 尹秀莲. 产耐热纤维素酶菌株的筛选及其酶学性质研究[J]. 安徽农业科学, 2010, 38(7): 3331-3332.
[15] 李强, 刘军, 周东坡, 等. 植物内生菌的开发与研究进展[J]. 生物技术通报, 2006, 3: 33-37.