FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (23): 23-26.

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Purification, Identification and Anti-A549 Cell Activity of Gypenosides from Thermally Processed Gynostemma pentaphyllum Leaves

  

  • Received:2011-10-07 Revised:2012-09-25 Online:2012-12-15 Published:2012-12-12
  • Contact: PIAO Xiang-Lan E-mail:xlpiao@163.com

Abstract: Objective: To extract, separate and identify anti-A549 cell constituents from thermally processed Gynostemma pentaphyllum leaves. Methods: Gynostemma pentaphyllum leaves were processed at 125 ℃ and 0.24 MPa for 3 h and subjected to heat reflux extraction with 80% ethanol for 3 h. Anti-A549 cell constituents in the extract were separated using HP-20 macroporous adsorption resin and a reversed-phase column by means of bioassay-guided separation and identified by 1H-NMR, 13C-NMR and ESI-MS. Results: Two dammarane-type saponins were obtained and identified as gypenoside L and gypenoside LI, respectively. Both gypenosides had a concentration-dependent inhibitory effect on A549 cells with IC50 values of (48.43 ± 1.33) μg/mL and (34.35 ± 0.88) μg/mL, respectively. Conclusion: Thermally processed Gynostemma pentaphyllum leaves have a notable inhibitory effect on A549 cells, which is associated with the presence of gypenoside L and gypenoside LI.

Key words: thermally processed Gynostemma pentaphyllum, lung cancer A549 cells, dammarane-type saponin, gypenoside L, gypenoside LI

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