FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (23): 278-282.

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Separation, Identification and Bioactivity of Anti-Tumor Components from Fermented Broth and Mycelia of Fungus Penicillium stoloniferum Derived from Salicornia herbacea

  

  • Received:2012-01-18 Revised:2012-11-02 Online:2012-12-15 Published:2012-12-12
  • Contact: zhihong xin E-mail:xzhfood@163.com

Abstract: Eight anti-tumor monomer compounds were isolated from the fermented broth and mycelia of the fungus Penicillium stoloniferum derived from Salicornia herbacea by bioassay-guided isolation method using silica gel column chromatography, Sephedex LH-20 chromatography, and semi-preparative HPLC. They were identified by physiochemical analysis and spectroscopic techniques as ergosta-7,9(11),22-trien-3-ol, ergosterol, spinasterol, 5α,8α-epidioxy-(22E,24R)-23-methylergosta-6,22-dien-3β-ol, tryptophan phenylalanine cyclic dipeptide, phenylalanine, dibutyl phthalate and diisobutyl phthalate. Only 5α,8α-epidioxy-(22E,24R)-23-methylergosta-6,22-dien-3β-ol, dibutyl phthalate and diisobutyl phthalate had strong cytotoxicity with IC50 values of 14, 34 μg/mL and 46 μg/mL, respectively, as evaluated by SRB method.

Key words: Salicornia herbacea fungi, fermentation products, structure identification, anti-tumor activity

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