Cyanidin-3-O-β-Glucoside Inhibits Lipoprotein Lipase Activity through Activating AMP-Activated Protein Kinase in Adipocytes

Abstract

Abstract: The major purpose of this study was to explore the effect of cyanidin-3-O-β-glucoside (Cy-3-g) on lipoprotein lipase(LPL) activity in adipocytes isolated from rats. It was found that Cy-3-g could inhibit LPL activity in adipocytes through activating pAMPK. This suggests that Cy-3-g may have a potential role against obesity through regulating lipid metabolism.

Key words: cyanidin-3-O-β-glucoside, adipocytes, lipoprotein lipase, AMP-activated protein kinase

CLC Number:

TQ936.16

References

[1] KUHNAU J. The flavonoids. A class of semi-essential food components: their role in human nutrition[J]. World Rev Nutr Diet, 1976, 24: 117-191.
[2] TSUDA T, HORIO F, KITOH J, et al. Protective effects of dietary cyanidin 3-O-beta-D-glucoside on liver ischemia-reperfusion injury in rats[J]. Arch Biochem Biophys, 1999, 368(2): 361-366.
[3] XIA M, HOU M, ZHU H, et al. Anthocyanins induce cholesterol efflux from mouse peritoneal macrophages: the role of the peroxisome proliferator-activated receptor {gamma}-liver X receptor {alpha}-ABCA1 pathway[J]. J Biol Chem, 2005, 280(44): 36792-36801.
[4] XU J.W, K. IKEDA, Y. YAMORI. Upregulation of endothelial nitric oxide synthase by cyanidin-3-glucoside, a typical anthocyanin pigment[J]. Hypertension, 2004, 44(2): 217-222.
[5] LI S.Q, Q.H. ZHANG. Advances in the development of functional foods from buckwheat[J]. Crit Rev Food Sci Nutr, 2001, 41(6): 451-464.
[6] XIA M, LING WH, Ma J, et al. Supplementation of diets with the black rice pigment fraction attenuates atherosclerotic plaque formation in apolipoprotein e deficient mice[J]. J Nutr, 2003, 133(3): 744-751.
[7] XIA M, LING WH, Ma J, et al. An anthocyanin-rich extract from black rice enhances atherosclerotic plaque stabilization in apolipoprotein E-deficient mice[J]. J Nutr, 2006, 136(8): 2220-2225.
[8] SASAKI R, NISHIMURA N, HOSHINO H, et al. Cyanidin 3-glucoside ameliorates hyperglycemia and insulin sensitivity due to downregulation of retinol binding protein 4 expression in diabetic mice[J]. Biochem Pharmacol, 2007, 74(11): 1619-1627.
[9] GUO H, LING W, WANG Q, et al. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. indica) on hyperlipidemia and insulin resistance in fructose-fed rats[J]. Plant Foods Hum Nutr, 2007, 62(1): 1-6.
[10] Qin Y, Xia M, Ma J, et al. Anthocyanin supplementation improves serum LDL- and HDL-cholesterol concentrations associated with the inhibition of cholesteryl ester transfer protein in dyslipidemic subjects[J]. Am J Clin Nutr, 2009, 90(3): 485-492.
[11] TSUDA T, HORIO F, UCHIDA K et al. Dietary cyanidin 3-O-beta-D-glucoside-rich purple corn color prevents obesity and ameliorates hyperglycemia in mice[J]. J Nutr, 2003, 133(7): 2125-2130.
[12] GOLDBERG I.J, M. MERKEL. Lipoprotein lipase: physiology, biochemistry, and molecular biology[J]. Front Biosci, 2001, 6: 388-405.
[13] FAN C, YAN J, QIAN Y, et al. Regulation of lipoprotein lipase expression by effect of hawthorn flavonoids on peroxisome proliferator response element pathway[J]. J Pharmacol Sci, 2006, 100(1): 51-58.
[14] HARDIE D.G, D. CARLING. The AMP-activated protein kinase--fuel gauge of the mammalian cell[J]. Eur J Biochem, 1997, 246(2): 259-273.
[15] LEONHARDT U, RITZEL U, OTTLEBEN M, et al. Circulating human hepatic lipase mRNA in patients with hepatocellular carcinoma and healthy controls[J]. Eur J Surg, 1999, 165(6): 539-542.
[16] VAN HARMELEN V, T. SKURK, H. HAUNER. Primary culture and differentiation of human adipocyte precursor cells. Methods Mol Med, 2005, 107: 125-135.
[17] BESSESEN D.H, C.L. RUPP, R.H. ECKEL. Trafficking of dietary fat in lean rats[J]. Obes Res, 1995, 3(2): 191-203.
[18] ZECHNER R. The tissue-specific expression of lipoprotein lipase: implications for energy and lipoprotein metabolism[J]. Curr Opin Lipidol, 1997, 8(2): 77-88.
[19] KIM S.J, C. NIAN, C.H. MCINTOSH. Activation of lipoprotein lipase by glucose-dependent insulinotropic polypeptide in adipocytes. A role for a protein kinase B, LKB1, and AMP-activated protein kinase cascade[J]. J Biol Chem, 2007, 282(12): 8557-8567.
[20] OHIRA M, MIYASHITA Y, MURANO T, et al. Metformin promotes induction of lipoprotein lipase in skeletal muscle through activation of adenosine monophosphate-activated protein kinase[J]. Metabolism, 2009, 58(10): 1408-1414.