Influence of Soybean Isoflavone on PKG and Raf-1 Expression in the Hippocampus of Rats with Alzheimer’s Disease

Abstract

Abstract: Objective: To explore the effect and possible mechanism of action of soybean isoflavone (SIF) on PKG and Raf-1 expression in the hippocampus of rats with Alzheimer’s disease (AD). Method: Female Wistar rats were randomly divided into shame operation model control and SIF high, medium and low-dose groups. AD rat models were established by bilateral hippocampus injection of Aβ. Learning-memory abilities in AD rats were observed by Morris water maze test. PKG content in the hippocampus was measured by ELISA and Raf-1 expression in the hippocampus of rats was detected by immunohistochemistrical SP method. Result: SIF significantly improved learning-memory abilities (P＜0.05) and decreased PKG content and Raf-1 expression in the hippocampus of AD rats (P＜0.05). Conclusion: SIF has anti-AD effects probably by decreasing PKG content and Raf-1 expression in the hippocampus of AD rats.

Key words: soybean isoflavone, Alzheimer’s disease, PKG, Raf-1

CLC Number:

R285.5

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