Antitumor Activity of Conjugated Linoleic Acid Converted from Linoleic Acid in Salicornia herbacea Seed Oil

Abstract

Abstract: Objective: To explore the in vitro antitumor activity of conjugated linoleic acid (CLA) converted from linoleic acid in Salicornia herbacea seed oil. Methods: CLA was chemically prepared from Salicornia herbacea seed oil containing 73.52% linoleic acid and analyzed for physiochemical properties and acute toxicity in mice. Meanwhile, its growth inhibitory effect on human tumor cells was measured by MTT assay. Results: The total content of c9, t11-CLA and t10, c12-CLA in the prepared CLA was 68.75%. Its physiochemical properties met relevant quality requirements. Acute toxicity test showed an LD50 larger than 20 g/kg, indicating its nontoxicity. Esophageal cancer cells EC109 and breast cancer cells HTB-22 were powerfully inhibited by this CLA with an IC50 of 111.9 μg/mL for the latter, but It had no inhibitory effect on cervical cancer cells HeLa cell and no toxicity to normal embryos renal cell 293T. Conclusion: The CLA prepared from Salicornia herbacea seed oil has a strong growth inhibition on some human tumor cells, but this inhibition is cell type specific.

Key words: Salicornia herbacea seed oil, conjugated linoleic acid, anti-tumor activity

CLC Number:

Q547

References

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