Abstract: The purpose of this study was to elucidate the promoting effect of Bonepep, bioactive peptides derived from egg yolk on bone metabolism and growth and its physiological mechanism. For this, mouse osteoblastic MC3T3-E1 cells, isolated rat osteoclast and rats were used and alkaline phosphatase (ALP) activity measurement, TRAP staining, bone erosion lacunae and BrdU staining were carried out in this study. It was found that Bonepep improved bone metabolism and growth through many different mechanisms such as promotion of osteoblast growth, inhibition of osteoclast growth and differentiation and enhancement of bone cell activity. 

Key words: Bonepep, bone metabolism, osteoblast, osteoclast