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Effect of Rhizoma Gastrodiae on Key Enenzyme Activities Involved in the Biosynthesis of Exopolysaccharides from Grifola frondosa in Submerged Culture

HE Zong-yi，WU Tian-xiang，XU Xiao-bao   

  1. 1. Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China；2. School of Life Sciences, Guizhou University,Guiyang 550025, China；3. School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China
  • Online:2013-06-15 Published:2013-06-03

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Abstract:

The effect of Rhizoma Gastrodiae on the biosynthesis of expolysaccharide (EPS) from Grifola frondosa in
submerged culture was studied. Total polysaccharide content was determined by phenol-sulphuric acid method. The results
showed that Rhizoma Gastrodiae at 7 g/L significantly promoted the biosynthesis of EPS in Grifola frondosa when compared
with blank control, increasing EPS yield from 3.72 g/L to 3.91 g/L. The measured activities of two key enzymes involved in
the synthesis of EPS indicated that after fermentation in the presence of Rhizoma Gastrodiae, PGI activity was decreased,
although α-PGM activity showed no obvious change.

Key words: Grifola frondosa；Rhizoma Gastrodiae；exopolysaccharide；PGI；&alpha, -PGM