Isolation of Staphylococcus aureus Bacteriophage-insensitive Mutant ATCC25923R and Adsorption Site of Phage JS01

doi:10.7506/spkx1002-6630-201311046

Abstract

Abstract:

The lysis of Staphylococcus aureus ATCC25923 by phage JS01 gave rise to a bacteriophage-insensitive mutant
(BIM, ATCC25923R). In order to understand the different sensitivity of ATCC25923 and ATCC25923R towards phage
JS01 as well as to determine the phage JS01 adsorption site on the cell surface of ATCC25923, the structures of teichoic
acids in these strains were analyzed by gas chromatography-mass spectrometry (GC-MS) and the effect of some reagents on
phage lysis was analyzed. ATCC25923R showed smooth colony with dark color when compared with ATCC25923. After
challenged with JS01, the OD600nm value of ATCC25923 culture decreased from 1.0 to 0.2 in 1 h, while the OD600nm value
of ATCC25923R culture increased continuously. A pentose component appeared in the teichoic acid of ATCC25923R,
but not in that of ATCC25923. Among glucose, N-acetylglucosamine (GlcNAc), and teichoic acid from ATCC25923 and
ATCC25923R, only GlcNAc could reduce the ability of JS01 to lyse ATCC25923, indicating that GlcNAc is the JS01
adsorption site on ATCC25923.

Key words: Staphylococcus aureus, bacteriophage, bacteriophage-insensitive mutants, teichoic acid, adsorption site

JIA Jing-jing，SHI Feng，WANG Li-ping，WANG Xiao-yuan. Isolation of Staphylococcus aureus Bacteriophage-insensitive Mutant ATCC25923R and Adsorption Site of Phage JS01[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201311046.

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Isolation of Staphylococcus aureus Bacteriophage-insensitive Mutant ATCC25923R and Adsorption Site of Phage JS01

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