Effect of Cyclocarya paliurus (Batal.) Iljinskaja Polysaccharide on the Proliferation and Differentiation of 3T3-L1 Preadipocytes

doi:10.7506/spkx1002-6630-201323058

Abstract

Abstract:

This study was conducted to investigate the effect of Cyclocarya paliurus (Batal.) Iljinskaja polysaccharide
on the proliferation and differentiation of 3T3-L1 preadipocytes. The crude polysaccharide extract of C. paliurus was
fractionated by using D301-R macroporous resin and DEAE-anion fiber resin, and a C. paliurus polysaccharide component
(CPC) was obtained. Gel permeation chromatography (GPC) and multi-angle laser light scattering (MALLS) were then used
to determine its molecular weight distribution. The proliferation and differentiation of 3T3-L1 preadipocytes treated with
different CPC concentrations were measured by MTT and oil red O staining, respectively. At the same time the glucose
consumption and extracellular glycerol content were determined. Results showed that CPC consisted of three different
components and their viscosity-average molecular weight (Mη) was determined to be 50300, 3951 g/mol and 1406 g/mol,
respectively. Within a certain concentration range, CPC affected the proliferation of preadipocytes in a concentrationdependent
manner. Compared with the control group, CPC could reduce the glucose consumption and extracellular glycerin
content of 3T3-L1 adipocytes with inhibition ratios of as high as 9.61% and 30.8% respectively. These observations reveal
that the C. paliurus polysaccharide is capable of promoting the proliferation of adipocytes in certain concentration range and
reducing glucose consumption and extracellular glycerin content.

Key words: Cyclocarya paliurus polysaccharide, 3T3-L1 adipocytes, viscosity average molecular weight, proliferation, differentiation

CLC Number:

Q946.8

LIU Yao，FU Ling-yun，YE Zhen-nan，LI Nan，WANG Wen-jun*. Effect of Cyclocarya paliurus (Batal.) Iljinskaja Polysaccharide on the Proliferation and Differentiation of 3T3-L1 Preadipocytes[J]. FOOD SCIENCE, 2013, 34(23): 291-295.

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