Abstract:

Fish bone powder-fish protein hydrolysate mixture was produced by mixing fish bone powder and fish proteinhydrolysates prepared from silver carp steak. With Wister male rats as a model, the calcium apparent absorptivity, calciumretention rate, femur biomechanical indicators, bone mineral density and other indicators were calculated to investigateeffect of fish bone powder particle size on the calcium bioavailability of fish bone powder-fish protein hydrolysate mixturein order to provide a theoretical basis for the use of fish bone powder for developing new calcium products. The resultsshowed that calcium apparent absorptivity, calcium retention rate, femur biomechanical indicators, bone mineral densityand other indicators (P ＜ 0.05), rather than body weight, liver index, spleen index or kidney index (P ＞ 0.05) indicatedsignificant differences among rats fed with different particle sizes of fish bone powder-fish protein hydrolysates mixture. Theindicators of small-particle-size (d ≤ 74 μm) fish bone powder-fish protein hydrolysate mixture were higher than those ofother experimental groups, calcium carbonate group and low calcium group, showing calcium apparent absorptivity, calciumretention rate, calcium content of femur and energy absorption of (47.84 ± 3.90)%, (44.94 ± 3.01)%, (24.18 ± 0.01)% and(7.52 ± 0.48) kg·mm, respectively, and the highest calcium bioavailability.

Key words: fish bone powder, fish protein hydrolysates, fish bone powder particle size, calcium bioavailability