Abstract: A method for the simultaneous determination of four Fusarium toxins in wheat flour including zenralenone, deoxynivalenol, T-2 and HT-2 toxins was established using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Samples were cleaned up with a MycoSep?226 multifunctional column, a DZT MS-PREP immunoaffinity column, which were both commercial products, and a polystyrene-divinylbenzene (PS-DVB) immunoaffinity column which was prepared in our laboratory, respectively. The recoveries of Fusarium toxins purified with MycoSep?226 ranged from 69.42% to 111.12% with relative standard deviations (RSDs) of 1.46%–13.24%, those obtained with PS-DVB ranged from 70.05% to 112.80% with RSDs of 3.42%–12.25%, and those obtained with DZT MS-PREP ranged from 88.01% to 107.31% with RSDs of 1.09%–14.42%. Immunoaffinity columns had the advantages of repeated use, less organic solvent consumption and high specificity. Moreover, PS-DVB could be a promising new immunoaffinity carrier.

Key words: Fusarium toxins, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), MycoSep?226 multifunctional column, polystyrene-divinylbenzene (PS-DVB) immunoaffinity column, DZT MS-PREP immunoaffinity column