Abstract Objective:To establish a systemic analysis method for rapidly determining content of aspartame in fruit jelly. Methods:The solid and semisolid foods were pretreated by special method,and then separated on C18 column with 0.1% tetrahydrofuran-acetonitrile as mobile phase and 210 nm detection wavelength. Result:The minimum detection limit is 2 mg/kg,and the average recoveries range from 97.0% to 99.2% with the relative standard deviations of 3.1%～5.3%. Conclusion:This method is simple and rapid. The aspartame in foods with complex components can be precisely analyzed with qualitative assay and quantitative assay.