Synthesis and Identification of Antigen of QCA and Preparation and Identification of Its Polyclonal Antibody

Abstract

Abstract: The QCA is coupled to BSA as an immunogen by the method of EDC,as well,the coating antigen(QCA-OVA) were produced by the method of MA.The immunogen and coating antigen have been identified by the method of ultraviolet full wavelength scanner.Polyclonal antibodies against QCA have been raised in four rabbits and purified by using a saturated ammonium sulfate method.The sensitivity and cross-reactivity of polyclonal antibody have been demonstrated in ELISA test.The results showed that the QCA antiserum have a very high degree of sensitivity and specificity.

Key words: carbadox, quinoxaline-2-carboxylic acid, artificial antigen, polyclonal antibody, indirect competitive ELISA

XIAO Zhen-Lei, XIE Zhong-Liang, HE Ji-Guo. Synthesis and Identification of Antigen of QCA and Preparation and Identification of Its Polyclonal Antibody[J]. FOOD SCIENCE, 2008, 29(5): 60-64.

[1]	ZHANG Biao, HUANG Na, ZHANG Wanli, WANG Shuo, SHENG Wei. Fluorescence Immunoassay Combined with Magnetic Separation Using Upconversion Nanoparticles as Label for the Detection of Tyramine in Food Samples [J]. FOOD SCIENCE, 2021, 42(14): 270-275.
[2]	JIN Dengpeng, ZHOU Xiong, LIU Huan, YANG Jiao, HUANG Zhuoquan, BU Lingling, KE Qianhua, LIU Chunhong. Proteomics Analysis of Breast Milk Whey Based on Immunoaffinity Chromatography and ProteoMiner [J]. FOOD SCIENCE, 2020, 41(24): 54-60.
[3]	ZHAO Jianhua, LI Haoxia, YIN Yue, WANG Yajun, LI Yanlong, FAN Yunfang, AN Wei, CAO Youlong. Cloning and Prokaryotic Expression of the Xylose Isomerase Gene (LbxylA) from Chinese Wolfberry (Lycium barbarum Linn.) Fruit and Preparation of Polycloal Antibody against the Fused Protein [J]. FOOD SCIENCE, 2019, 40(10): 77-83.
[4]	DAI Jieyu, JIANG Yonghua, ZHANG Yujie, WANG Haojie, LIU Xiaoran, LIU Cuihua, REN Xiaolin. Prokaryotic Expression of MdHB1, a Transcription Factor from Malus domestica and Preparation of Polyclonal Antibody against It [J]. FOOD SCIENCE, 2018, 39(8): 69-76.
[5]	WANG Jing, MA Ningning, SONG Yang. Preparation of Artificial Antigen and Polyclonal Antibody for the Detection of Heterocyclic Amines [J]. FOOD SCIENCE, 2017, 38(4): 45-50.
[6]	WANG Jing, WANG Yao, WANG Fangyu, DENG Ruiguang, HU Xiaofei, YU Qiuying, HAO Junfang, XING Yunrui, HOU Yuze. Preparation of Artificial Antigen and ELISA Identification of Rabbit Polyclonal Antiserum for the Detection of Glyphosate [J]. FOOD SCIENCE, 2017, 38(4): 65-69.
[7]	ZHU Fan, LI Huanting, WANG Hong, YANG Jinyi, XU Zhenlin, LEI Hongtao, XIAO Zhili, SUN Yuanming, SHEN Yudong. Development of Indirect Competitive Chemiluminescene Enzyme Immunoassay (icCLEIA) Method for Determination of Iso-Tenuazonic Acid in Fruit Juice and Tomato Sauce [J]. FOOD SCIENCE, 2017, 38(4): 250-254.
[8]	WANG Xiaofen, YAN Aiping, TAN Ting, MAO Xuejin, LIU Junjun, GUO Lan, WAN Yiqun. Synthesis and Identification of Stable Artificial Paclobutrazol Antigen [J]. FOOD SCIENCE, 2016, 37(7): 134-139.
[9]	LAI Dan, ZHENG Shengwu, ZHAO Suqing. Synthesis of Artificial Antigens for Immunoassay of Four Phthalate Esters [J]. FOOD SCIENCE, 2016, 37(5): 114-120.
[10]	LI Peipei, ZHANG Xiaojun, YAN Zhongyong, CHEN Si, LUN Lili, GAO Xuehui, LIU Wenjing. Determination of Methyl-3-quinoxaline-2-carboxylic Acid in Fish and Shrimp Samples by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with Immunoaffinity Column Cleanup [J]. FOOD SCIENCE, 2016, 37(24): 257-261.
[11]	DING Chengchao, XIE Manman, ZENG Haijuan, LIU Qing. Gene Sequence Analysis and Polyclonal Antibody Preparation of Outer Membrane Protein, Ompk, in Vibrio [J]. FOOD SCIENCE, 2016, 37(23): 141-146.
[12]	XIAO Shimei, YAN Aiping, XIAO Fang, WAN Yiqun, GUO Lan. Synthesis and Identification of Artificial Antigens of Forchlorfenuron [J]. FOOD SCIENCE, 2016, 37(21): 183-188.
[13]	WANG Nannan, LI Yansong, MENG Xingyu, ZHOU Yu*, QIAO Bin, SUN Yu, HU Pan, LU Shiying, REN Honglin, LIU Zengshan. Preparation and Characterization of Fluorene Complete Antigen and Anti-fluorene Polyclonal Antibody [J]. FOOD SCIENCE, 2015, 36(9): 108-112.
[14]	LIU Huihui1, XU Yingjiang1, SONG Xiangjun1, ZOU Rongjie2, DENG Xuxiu2, HAN Dianfeng1, TIAN Xiuhui1, AN Honghong3, GONG Xianghong1,*. Metabolism of Mequindox in Juvenile Apostichopus japonicus and Safety Evaluation of Its Metabolites for Consumption of Adult Apostichopus japonicus [J]. FOOD SCIENCE, 2015, 36(1): 214-219.
[15]	WU Chun-sheng, MA Liang, HU Yuan-yuan, ZHANG Yu-hao. Recent Progress in the Preparation of Artificial Antigen of Mycotoxins [J]. FOOD SCIENCE, 2014, 35(5): 239-245.

Synthesis and Identification of Antigen of QCA and Preparation and Identification of Its Polyclonal Antibody

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments