FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (5): 101-103.

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Extraction of Citrus suavissima Polysaccharide and Its Effect of Scanvenging Oxygen Radical

 WU  Jian-Zhang, YE  Hui, XIANG  Zheng, YANG  Xiao-Feng   

  1. 1.School of Pharmacy,Wenzhou Medical College;2.Faculty of Biochemistry,Wenzhou Medical College
  • Online:2008-05-15 Published:2011-08-26

Abstract: Objective:To extract polysaccharide from Citrus suavissima and analyze its effect of cleaning oxygen radical.Method:The polysaccharide was extracted with hot water at 100 ℃ and precipitated by alcohol.The protein in the extractions was removed by the sevage method.Test were also done with the extraction material,including the determination of sugar,the analysis of polysaccharide behaviour,and the determination of the activities of cleaning oxygen racial.Result:the extraction ratio was 1.55%,and its total sugar content was 52.07 %.IR showed the absorption peak of sugar.In the determination of the activities of cleaning O2· and ·OH,its IC50 was 880 μg/ml and 1280 μg/ml respectively.Conclusion:CSPS has the effect of cleaning oxygen radical.Its activity is positively relative with dosage in certain range.

Key words: Citrus suavissima, polysaccharide, hydroxyl free radical, superoxide anion free radical