Study on Separation of Peptides Derived from Oreochromis niloticus Skin with Ultra-filtration Technology and Its Antioxidative Activity

Abstract

Abstract: Peptides derived from TSP-I was separated by ultra-filtration technology,the effects of pressure,temperature and concentration of peptides on ultra-filtration permeate flux,permeate increment and membrane efficiency.The results showed that the optimum conditions are:5% TSP-I,pressure 28 Psi and time 40 min.Under the condition,ultra-filtration permeate flux is 9.98 LHM,ultra-filtration permeate increment is 2.68 g/10min and membrane efficiency is 3.41 g/m2·min.The capacities on scavenging O2·,·OH,DPPH·and H2O2 of VC and TSP-II were investigated by spectrophotometry.Study informed that TSP-II had a certain extent antioxidation,the order of scavenging ability is H2O2>·OH >O·2>DPPH·.On scavenging H2O2,0.80 mg/ml TSP-II is equivalent to 0.03 mg/ml VC.

Key words: tilapia(Oreochromis niloticus)skin, collagen polypeptide, radicalanti-oxidizing action, spectrophotometry

LIU Cheng-Mei, LIANG Han-Ying, LIU Wei, WAN Jie. Study on Separation of Peptides Derived from Oreochromis niloticus Skin with Ultra-filtration Technology and Its Antioxidative Activity[J]. FOOD SCIENCE, 2008, 29(5): 227-230.

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Study on Separation of Peptides Derived from Oreochromis niloticus Skin with Ultra-filtration Technology and Its Antioxidative Activity

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