Abstract: The technique of hydrolysis of soybean isoflavones by β-glycosidas"eAmano"was studied in this study.The different influencing factors during the process of enzyme hydrolysis were studied through single factor tests.The reaction conditions of hydrolysis by β-glycosidas"eAmano" were optimized through orthogonal test.The optimal reaction conditions of hydrolysis of daidzin were:time 140 min,the concentration of enzyme 33μg/ml,pH 4.5,temperature 50 ℃.The optimal reaction conditions of hydrolysis of genistin were:time 140 min,the concentration of enzyme 33μg/ml,pH 4.5,temperature 60 ℃.The identified test proved the hydrolysis ratio of daidzin attained 98.2% and the hydrolysis ratio of genistin attained 94.2% under the optimal conditions.

Key words: soybean isoflavones； &beta, -glycosidase", Amano", ； hydrolysis； HPLC；