FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (5): 269-272.

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An Efficient and Rapid Method for DNA Extraction and Species Identification from Sea Cucumber Nutrient Solution

 LIANG  Jun-Ni, XUE  Chang-Hu, ZHAO  Yu-Ran, TANG  Qing-Juan, XU  Jia-Chao, YANG  Wen-Ge   

  1. .College of Food Science and Engineering,Ocean University of China;2.Faculty of Life Science and Biotechnology,Ningbo University
  • Online:2008-05-15 Published:2011-08-26

Abstract: Mitochondria DNA of sea cucumber were extracted from the sea cucumber nutrient solution with optimized method.The quantitative and qualitative analyses of DNA solution were performed with the calculation of A260/A280,Agarose gel electrophoresis,and PCR.The results showed that our method of DNA extraction and species identification from the sea cucumber nutrient solution was more efficient and rapid than traditional methods.This study will be helpful for quicker,more economical,and dependable species identification of the sea cucumber.

Key words: sea cucumber nutrient solution, DNA extraction, species identification