FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (5): 292-294.

Previous Articles     Next Articles

Study on Purification and Properties of Soybean Esterase

 WEN  Yan-Xia, LAN  Wen-Li, LI  Jian-Ke   

  1. 1.Department of Petrochemical Engineering,Yinchuan University;2.Ningxia Fengyou Chemical Company;3.Department of Food Engineering,Shaanxi Normal University
  • Online:2008-05-15 Published:2011-08-26

Abstract: The purification method and properties of plant esterase(P-E) from soybean were studied.Results showed that the electrophoresis purity P-E can be obtained by extraction with phosphate buffer(0.3 mol/L,pH 7.0) extraction,differential centrifugation,ammonium sulfate deposition and ion-exchange chromatography.The optimal temperature,pH and Km of P-E were also determined respectively.

Key words: plant esterase, purification, optimal temperature, optimal pH, Michaelis constant