FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (5): 337-340.

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Construction of cDNA Library of Camellia ptilophylla Chang

 WEN  Hai-Tao, CHEN  Zhong-Zheng, ZHAO  Liang, GUO  Jian, LI  Bin, JIANG  Yue-Ming   

  1. 1.College of Food Science,South China Agricultural University,Guangzhou 510642,China;2.College of Food Science,Biotechnology and Environmental Engineering,Zhejiang Gongshang University
  • Online:2008-05-15 Published:2011-08-26

Abstract: In this study,cDNA library was constructed successfully from the specie of Camellia ptilophylla Chang with a low content of caffeine through using a series of ZAP kits that bought from the company of Stratagene.The titer and recombinant rate of this library were 6.0×105pfu/ml and 97.3 % respectively,the insert size with 1.0~2.0kb occupied 70% of the whole library,matching the requirement of high quality library.The titer and recombinant rate of amplification library were 1.9×109pfu/ml and 99.1%,respectively.

Key words: Camellia ptilophylla Chang, cDNA library, library titer