FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (5): 337-340.
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WEN Hai-Tao, CHEN Zhong-Zheng, ZHAO Liang, GUO Jian, LI Bin, JIANG Yue-Ming
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Abstract: In this study,cDNA library was constructed successfully from the specie of Camellia ptilophylla Chang with a low content of caffeine through using a series of ZAP kits that bought from the company of Stratagene.The titer and recombinant rate of this library were 6.0×105pfu/ml and 97.3 % respectively,the insert size with 1.0~2.0kb occupied 70% of the whole library,matching the requirement of high quality library.The titer and recombinant rate of amplification library were 1.9×109pfu/ml and 99.1%,respectively.
Key words: Camellia ptilophylla Chang, cDNA library, library titer
WEN Hai-Tao, CHEN Zhong-Zheng, ZHAO Liang, GUO Jian, LI Bin, JIANG Yue-Ming. Construction of cDNA Library of Camellia ptilophylla Chang[J]. FOOD SCIENCE, 2008, 29(5): 337-340.
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