Determination of Furosine in Royal Jelly by Ion-Pair Reversed Phase Liquid Chromatography

Abstract

Abstract: The determination of furosine in royal jelly by ion-pair reversed phase liquid chromatography was carried out.The separation of furosine was accomplished on Atlantis C18 column.Acetonitrile/water(5mmol/L sodium heptane sulfonate)/formic acid(20:79.8:0.2)was used as the mobile phase.It was detected by a UV detector at 280 nm.The linear range was 0.25～50 mg/L with equation of y=0.2818x+0.0271(r=0.9997).The method detection limit of furosine was 11.9 mg/100g protein.The average recoveries was 96.94%.The method is satisfactory for determing furosine in royal jelly.

Key words: royal jelly, furosine, ion-pair reversed phase liquid chromatography

ZHOU Xiao, XUE Xiao-Feng, WU Li-Ming, ZHAO Jing. Determination of Furosine in Royal Jelly by Ion-Pair Reversed Phase Liquid Chromatography[J]. FOOD SCIENCE, 2008, 29(5): 370-372.

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Determination of Furosine in Royal Jelly by Ion-Pair Reversed Phase Liquid Chromatography

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