Prediction of B-cell Linear Epitopes in Egg Allergen Ovotransferrin

Abstract

Abstract: In order to predict B-cell linear epitopes of egg allergen ovotransferrin (OVT), the antigen index, hydrophilicity, and surface accessibility and flexibility of egg OVT were predicted using Jameson-Wolf, Kyte-Doolittle, Emini and Karplus-Schulz methods, respectively. The secondary structure of OVT was predicted by Chou-Fasman and Deleage-Roux methods based on the amino acid sequence of egg OVT derived from Genbank. Based on the above comprehensive analysis, 7 possible B-cell linear epitope candidates in egg OVT were predicted to locate in the regions of 174－181, 215－222, 231－240, 288－294, 332－344, 415－429 and 547－555. These results are useful for fine mapping of B-cell epitopes in egg OVT.

Key words: ovotransferrin, B-cell linear epitope, egg allergy, prediction

CLC Number:

Q518.1

References

[1] MINE Y, RUPA P. Immunological and biochemical properties of egg allergen[J]. World Poultry Sci J, 2004, 60: 321-330.
[2] HITOSHI A, ROBERT M, YASUTO K, et al. Utility of ovomucoid-speci?c IgE concentrations in predicting symptomatic egg allergy[J]. J Allergy Clin Immunol, 2008, 122(3): 583-588.
[3] ANET J, BACK J F, BAKER R S, et al．Allergens in the white and yolk of hen’s egg: A study of IgE binding by egg proteins[J]．Int Arch Allergy Appl Immunol，1985，77：364-371.
[4] WILLIAMS J, ELLEMAN T C, KINGSTON I B, et al. The primary structure of hen ovotransferrin[J]. Eur J Biochem. 1982, 122: 297-303.
[5] SUZUKI M, FUJII H, FUJIGAKI H, et al. Lipocalin-Type Prostaglandin D Synthase and Egg White Cystatin React with IgE Antibodies from Children with Egg Allergy[J]. Allergology International. 2010, 59: 175-183.
[6] BARLOW D J, EDWARDS M S, THORNTON J M. Continuous and discontinuous protein antigenic determinants[J]. Nature, 1986, 322: 747-748.
[7] 杨霞, 刘凯军, 申子刚, 等. 人I zumo蛋白的二级结构及B细胞抗原表位预测[J]. 中国免疫学杂志, 2010, 1: 37-40.
[8] 勾蓝图, 杨金亮. 基于生物信息学的ECSM2胞外区B细胞表位预测[J]. 免疫学杂志, 2010, 26(2): 156-160.
[9] 胡纯秋, 高金燕, 罗春萍, 等. 花生过敏原Ara h 2.02二级结构和B细胞抗原表位预测[J]. 食品科学, 2009, 30(21): 13-15.
[10] 朱盼, 陈红兵, 胡纯秋, 等. 基于表位预测的花生过敏原Ara h 6免疫交叉反应性研究[J]. 食品科学, 2010, 31(17): 318-322.
[11] JAMESON B A, WOLF H. The antigenic index: a novel algorithm for predicting antigenic determinants[J]. Comput Appl Biosci, 1988, 4(1): 181-186.
[12] KYTE J, DOOLITTLE R F. A simple method for displaying the hydropathic character of a protein[J]. J Mol Biol, 1982, 157(1): 105-132.
[13] EMMINI E A, HUGHES J V, PERLOW D S, et al. Induction of hepatitis a virus-neutralizing antibody by a virus-specific synthetic peptide[J]. J Viorol, 1985, 55(3): 836-839.
[14] KARPLUS P A, SCHULZ G E. Prediction of chain flexibility in proteins[J]. Naturwissenschaften, 1985, 72(4): 212-213.
[15] CHOU P Y, FASMAN J. Prediction of the secondary Structure of proteins from their amino acid sequences[J]. Adv Enzymology, 1978, 47: 45-49.
[16] DELEAGE G, ROUX B. An algorithm for protein secondary structure prediction based on class prediction[J]. Protein Eng, 1987, 1(4):289-294.
[17] 李海侠, 毛旭虎. 蛋白质抗原表位研究进展[J]. 微生物学免疫学进展, 2007, 35(1): 54-58.
[18] 来鲁华. 蛋白质的结构预测与分子设计. 1 版［M］. 北京：北京大学出版社，1993：49.
[19] 程健, 张佩, 马鸣潇, 等. 中蜂囊状幼虫病毒LN-QY株VP1蛋白的空间结构与B细胞抗原表位预测[J]. 中国生物制品学杂志, 2011, 24(3): 280-284.
[20] 李晋涛, 陈正琼, 梁志清, 等. Eppin抗原的二级结构分析B细胞表位预测[J] .第三军医大学学报, 2008, 30 (24): 2254-2257.