FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (1): 103-105.doi: 10.7506/spkx1002-6630-201201020

• Basic Research • Previous Articles     Next Articles

Anti-Bacillus cereus Mechanisms of Fructus Mume Extract

LIU Meng-yin,LIU Fang,ZHOU Tao,ZHU Yong-zhi,XU Wei-min   

  1. 1. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. Ginling College, Nanjing Normal University, Nanjing 210097, China
  • Received:2011-03-14 Revised:2012-01-08 Online:2012-01-15 Published:2012-01-12
  • Contact: LIU Meng-yin E-mail:dlhlh2000@yahoo.com.cn

Abstract:

The objective of the present study was to investigate the mechanisms for the inhibition of Bacillus cereus by Fructus Mume extract. The effect of Fructus Mume extract on the integrity of cell wall was studied by determining the content of alkaline phosphatase (AKP). The contents of extracellular proteins were assayed by Bradford method. Cell membrane permeability was examined based on changes in electrical conductivity. The effect of Fructus Mume extract on the synthesis of bacterial proteins was analyzed by SDS-PAGE. The results indicated that Fructus Mume extract could damage the structures of cell wall and cell membrane, causing an increase in cell membrane permeability and the release of intracellular contents. Fructus Mume extract could also affect the synthesis of bacterial proteins and block protein expression in bacteria.

Key words: Fructus Mume, nantibacterial mechanism, Bacillus cereus

CLC Number: