FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (8): 313-317.

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Studies on the Determination of Total Saponin of the Momordica. charantia Var. abbreviata Ser

 GU  Xiao-Hong, ZHAO  Hai-Wen, TANG  Jian, WANG  Li-Ping   

  1. 1.Analysis Center Southern Yangtze University;2.School of Food Science andTechnology, Southern Yangtze University
  • Online:2005-08-15 Published:2011-09-19

Abstract: A spectrophotometric method was established by using ginseng saponin as standard in 546nm for the quantitativeanalysis of the total saponins in the Momordica. charantiaV ar.a bbreviata Ser. Colorimetric system was established that the samplereacted with 5% vanillin-acetic acid glacial solution and 0.8ml the perchloric acid at 60℃ for 15min, then it turned to amaranth.The analytical precision was good, RSD was 0.39%, the average recovery was 102.56%, the standard error was 6.12, CV was 5.97%.

Key words: Momordica. charantia Var. abbreviata Ser, saponin, determination