FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (3 ): 243-248.doi: 10.7506/spkx1002-6630-200903055

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Optimization of Fermentation Conditions for Methionine Production by Ethionine-resistant Mutant

LIN Xiao-qiu,FENG Qi,ZHANG Dong-huan,MIN Wei-hong*   

  1. (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
  • Received:2008-10-29 Online:2009-02-01 Published:2010-12-29
  • Contact: MIN Wei-hong* E-mail:minwh2000@163.com

Abstract:

This study aimed to optimize the conditions of nutrition and cultivation of ethionine-resistant mutant E31. The results showed the best nutrition conditions are 11% glucose, 0.8% urea, 0.08% MgSO4·7H2O, 3 μg /100ml biotin, the best cultivation conditions are pH 6,fermentation temperature 33℃, inoculation size 8% and fermentation time 84 h, After the opimization, the yield of methionine is 2.086 g/L, 0.607g/L higher than that before the optimization.

Key words: methionine, ethionine resistant mutant, fermentation

CLC Number: