Optimization of Cellulase Extraction Conditions for Crude Polysaccharides from Perilla frutescens crispa by Response Surface Methodology

Abstract

Abstract: Abstract: Perilla polysaccharide is a functional ingredient. The cellulose extraction process of polysaccharides from perilla was Optimized in this paper. Diffient drying methods which influence the polysaccharide content were studied, including sunshine drying, natue wind drying, vacuum drying, blast drying and microwave drying. vacuum drying was the best pre-process method for polysaccharide extraction. Based on the experiment of single factor, the mathematical regression model was established about the dependent variable (extraction yield of polysaccharides) and independent variables (adding content of cellulose, extraction temperature, extraction time) through Box-Benhnken center combination design and response surface analysis. The optimum extraction conditions were as follow: adding content of cellulose of 1771.85U/g, extraction temperature of 53.7℃ and extraction time of 36.2min. Under these condition, the practical yield of polysaccharides (17.91mg/g) was consistent with the predictive yield of polysaccharides (18.21mg/g).

CLC Number:

TS219

References

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