FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (1): 234-238.

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Mutation Breeding of High-Yield Kojic Acid-Producing Strains by UV Irradiation Combined with N+ Implantation

  

  • Received:2011-10-08 Revised:2012-12-17 Online:2013-01-15 Published:2013-01-07

Abstract: Aspergillus oryzae CICC-2336 was found to the best strain for the production of kojic acid followed by Huniang 3.811, AS 3.866, Yu3.811 and CICC-2337. A high-yield kojic acid-producing strain designated as N11 was obtained from the starting strain CICC-2336 by UV irradiation combined with N+ implantation. After the sixth passage, mutant N11 remained stable and its kojic acid production remained basically unchanged. The production of kojic acid was 24.12 g/L after 7 d of fermentation at 30 ℃ and a shaking speed of 180 r/min with an inoculum size of 15%, which was 101% higher than that of the starting strain.

Key words: Aspergillus oryzae, kojic acid, UV-induced mutation, N+ implantation-induced mutation, subculture

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