FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (1): 300-302.

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Isolation and Antitumor Activity of Proteins from Allium chinense Bulbs

  

  • Received:2011-10-19 Revised:2012-12-06 Online:2013-01-15 Published:2013-01-07

Abstract: Allium chinense bulbs were extracted with pH 7.5 100 mmol/L Tris-HCl buffer solution and salted out with ammonium sulfate at different degrees of saturation to obtain total proteins. The antitumor activity of total protein extracts against mouse B16 melanoma cells and Meth-A cells was tested. Protein extracts obtained with 20%—80% saturated ammonium sulfate had potent cytotoxicity against both tumor cells and protein extract with the most potent cytotoxicity was obtained with 80% saturated ammonium sulfate, which mainly consisted of alliinase, heat shock protein, lectin, cysteine synthase and members of the tumor necrosis factor receptor super family as shown by LC-MS analysis.

Key words: Allium chinenese, protein, melanoma, anti-tumor, LC-MS

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