Synthesis and Characterization of Ractopamine Antigen Linked by 4-Methylbenzoic Acid

Abstract

Abstract: The mixed anhydride method was used to prepare artificial antigen through the conjugation between ractopamine (RAC) and bovine serum albumin (BSA) in the presence of 4-chloromethyl benzoic acid (CBA). The prepared artificial antigen was identified by IR, UV and SDS-PAGE. RAC-CBA was also conjugated with OVA to form a coating antigen. The affinity and inhibitory activity of the coating antigen were measured. This study suggests that CBA can be used to prepare artificial RAC antigens. Meanwhile, CBA conjugated with RAC to form coating agent improves the sensitivity of ELISA.

Key words: ractopamine, 4-chloromethyl benzoic acid, ELISA sensitivity, artificial antigens

CLC Number:

S 859.84

References

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