FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (5): 202-206.doi: 10.7506/spkx1002-6630-201305042

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Screening and Breeding of a Crypthecodinium cohnii Strain with High Productivity of Docosahexaenoic Acid and Optimization of Its Fermentation Conditions

SUN Zhong-guan1,2,LIU Yong1,*,YAO Jian-ming3,LI Jun4,ZHU Su-mei4   

  1. 1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China;2. College of Life Science, Zaozhuang University of Technology, Zaozhuang 277160, China;3. Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China;4. Anhui Keju Green Energy Co. Ltd., Hefei 230031, China
  • Received:2011-11-17 Revised:2013-01-17 Online:2013-03-15 Published:2013-04-16
  • Contact: LIU Yong E-mail:liuy99999@163.com

Abstract: In order to improve the productivity of docosahexaenoic acid, the mutagenesis was carried out through nitrosoguanidine (NTG) induction by using Crypthecodinium cohnii ATCC30556 as the original strain. The mutant LS1057 was selected through high-temperature (30 ℃) cultivation using docosahexaenoic acid content in total fatty acids as the evaluation index. The content of docosahexaenoic acid was increased from 25.78% to 32.02%. The fermentation conditions for LS1057 were optimized by single-factor tests to be pH 6.5, fermentation temperature of 26 ℃, shaking speed of 150 r/min and cultivation time of 216 h. Under the optimal conditions, the biomass of cultivated LS1057 could reach up to 14.06 g/L with total lipid of 3.14 g/L and docosahexaenoic acid of 1.057 g/L, which exhibited the enhancement by 21.31%, 69.73% and 121.59% when its parent strain.

Key words: docosahexaenoic acid (DHA), nitrosoguanidine, Crypthecodinium cohnii, mutagenesis, fermentation conditions

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