FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (2): 204-208.

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Determination of sulfonamide in vegetable by solid phase extraction- high performance liquid phase chromatography

  

  • Received:2011-11-14 Revised:2012-12-07 Online:2013-01-25 Published:2013-01-15

Abstract:

A solid extraction column was prepared with graphene as adsorbent. Analytical method for the determination of six sulfonamides was developed based on graphene solid-phase extraction (SPE) coupled with high-performance liquid-phase chromatography. Ultraviolet detection was used for qualitative and quantitative analysis at 270 nm. Factors that may influence the extraction efficiency including the kind of adsorbents, the kind and volume of eluent, pH of sample solutions and sample volume were optimized in detail. Under the optimized conditions, there was good linearity in the concentration range of 0.1-100 μg/L with the correlation coefficients of 0.990-0.997. Limits of detection were in the range of 6.3-21.1 ng/L (S/N = 3). The precisions of proposed method were in the range of 2.12-4.75%. The method was applied to the analysis of vegetable samples with recoveries of 78.5% -103.2%.

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