FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (2): 231-235.

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Content Determination of Hyperoside and Total Flavonoids in Leaves of Rosa davurica Pall.

  

  • Received:2011-08-05 Revised:2012-07-23 Online:2013-01-25 Published:2013-01-15

Abstract:

Abstract: Purpose: To establish the determination method of total flavonoids and hyperoside in leaves of Rosa davurica Pall..Method:UV-spectrophotometric method was applied to determine the content of total flavonoids at the wave length of 418 nm using rutin as reference substance. The content of hyperin was determined with high performance liquid chromatography(HPLC) using Shim-pack VP-ODS C18 (150mm×4.6mm,5μm) column and CH3OH-0.5% H3PO4 (45:55)as gradient elution mobile phase. The UV detection wave-length, the flow rate and the column temperature were 360nm,1.0 mL/min, 40℃ respectively. Result: Total flavonoids and hyperoside have shown good linear relationship in the range of 0.02~0.12mg/mL(R=0.9996)and 0.192~1.152μg(R=0.9993).The average recovery rate and RSD of total flavonoids and hyperoside were100.87%,100.36%, 2.79% and 2.26% respectively. Conclusion: The determination method offered in the paper is characterized with celerity,accuracy and easy operation, which is fit for the content determination of total flavonoids and hyperoside in leaves of Rosa davurica Pall..

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