Preparation and Application of Hydrogen Dioxide Biosensor Based on Microelectrode Array of SiO2 Cavity

doi:10.7506/spkx1002-6630-201306004

Abstract

Abstract: A new biosensor for the detection of H2O2 was prepared by immobilizing microperoxidase-11 (MP-11) onto silicon dioxide (SiO2) cavity array directly. Polystyrene (PS) particle array was constructed on indium-tin oxide (ITO) electrode surface by Langmuir-Blodgett technique. The silicon dioxide cavity array was prepared by Langmuir-Blodgett technique using PS particle array as the template. Using MP-11 as a model protein, MP-11/SiO2 cavity array-ITO electrode was prepared by adsorbing MP-11 into SiO2 cavity. The fast response of the biosensor against H2O2 was observed. A linear relationship between current response and H2O2 concentration ranging from 7.06 × 10-6 to 4.02 × 10-2 mol/L was achieved with a detection limit of 3.0 × 10-7 mol/L. The apparent Michaelis-Menten constant was 0.916 mmol/L. This established method is satisfactory for the determination of hydrogen peroxide in food samples with a recovery rate varying from 94% to 97%.

Key words: hydrogen peroxide, sol-gel technique, silicon dioxide cavity, microelectrode array, microperoxidase-11, biosensor

CLC Number:

References

[1] 刘海峰. 快速检测食品中过氧化氢残留的生物传感方法的建立[D]. 上海: 上海交通大学, 2009.
[2] 林倩, 张菊, 王晓芳, 等. 食品级过氧化氢的应用及其净化技术[J]. 食品科学, 2006, 27(10): 626-649.
[3] 文静, 张西, 陈曦, 等. 体内羟自由基的检测方法[J]. 食品科学, 2004, 25(10): 351-356.
[4] 朱玲. 工业过氧化氢含量测试方法的探讨[J]. 安微化工, 2002, 120(6): 45-46.
[5] 张国芳, 陈洪渊. 流动注射胶束电化学发光测定过氧化氢的研究[J]. 分析科学学报, 2001, 17(1): 1-5.
[6] Janasek D, Spohn U. The analysis of hydrogen peroxide in acequieous solution[J]. Sensor and Actuators B, 1998, 51(1): 107-113.
[7] 戴春霞. 基于硫胺素荧光增强的可更新液滴荧光法用于痕量过氧化氢的测定[J]. 精细化工中间体, 2005, 35(6): 51-55.
[8] 许利君, 王凯雄, KANG Joon-wu, 等. 过氧化氢测定方法研究及水处理过程中过氧化氢自动连续检测系统的设计构思[J]. 浙江大学学报(理学版), 2003, 30(3): 303-309.
[9] Liang Aihui, Zhou Sumei, Jiang Zhiliang. A simple and sensitive resonance scattering spectral method for determination of hydroxyl radical in Fenton system using rhodamine S and its application to screening the antioxidant[J]. Talanta, 2006, 70: 444-448.
[10] Lian Wenping, Wang Li, Song Yonghai, et al. A hydrogen peroxide sensor based on electrochemically roughened silver electrodes[J]. Electrochimica Acta, 2009, 54 (18): 4334-4339.
[11] 白红艳，宋利. 过氧化氢电流型传感器检测方法的研究进展[J]. 广西轻工业, 2011, 4(4): 86-87, 28(1): 92-95.
[12] 邓培红, 张军, 黎拒难. 多壁碳纳米管修饰炭黑微电极同时测定多巴胺和抗坏血酸[J]. 分析实验室, 2009.
[13] 孙康, 王知彩, 李仲辉. SiO2/Nafion杂化膜固定酶的过氧化氢生物传感器[J]. 分析测试学报, 2011, 29(11): 1185-1189.
[14] 王刊, 王菊芳. 辣根过氧化物酶在亲水性离子液体中的活性与稳定性研究[J]. 分子催化, 2009, 23(1): 73-77.
[15] Razumas V, Kazlauskaite J, Ruzgas T, et al. Bioelectro-chemistry of microperoxidases[J]. Bioelectrochem Bioenerg, 1992, 28(01-02): 159-176.
[16] Jiang Huijun, Mercier P, Wang S, et al. Direct electrochemistry and electrocatalytic activity of microperoxidase-11 immobilized on chitosan wrapped single-walled carbon nanotubes[J]. Journal of functional materials and devices, 2009, 15(2): 132-142.
[17] 周丽娟, 二氧化硅球腔微电极阵列以及复合磁性纳米氧化铁的制备与应用[D]. 苏州: 苏州大学, 2011.
[18] 王凤彬, 郑军伟, 李晓伟. 金电极表面聚赖氨酸固定微过氧化物酶-11的电化学研究[J]. 高等学校化学学报, 2003, 24(12): 2268-2270.
[19] Bard A J, Faulkner L R. Electrochemical methods, fundamental and applications[M]. 2nd ed. New York: John Wiley Sons, 2001: 594.
[20] 王全林, 杨宝军, 吕功煊. 碳糊电极上无机膜固载血红蛋白的直接电化学[J]. 高等学校化学学报, 2003, 24(9): 1561-1566.
[21] Liu Ying, Wang Mingkui, Zhao Feng, et al. Direct electron transfer and electrocatalysis of microperoxidase immobilized on nanohybrid film[J]. ElectroanalyticalChemistry, 2005, 81(01): 1-10.