FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (2): 179-181.

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High-performance Liquid Chromatography with Nano Quantity Analyte Detector for the Determination of D-Xylose

  

  • Received:2011-11-01 Revised:2012-11-30 Online:2013-01-25 Published:2013-01-15

Abstract:

Abstract:Objective:To develop a fast, sensitive, accurate high-performance liquid chromatography(HPLC) with nano quantity analyte detector (NQAD) for the determination of D-xylose. Method: The chromatographic separation was achieved with an amino column using a mobile phase composed of a mixture of acetonitrile and water (73:27, V/V) at a flow rate of 0.8 mL/min. The NQAD drift tube temperature was set at 50℃ and the N2 pressure at 30psi. Result: The linear range of D-xylose was between 10μg/mL and 120μg/mL (R2=0.9998). The detection limit was 0.7μg/mL. The average spike recovery was 101.97%, with a relative standard deviation (RSD) of 0.88%. Conclution:This method is sensitive, accurate and repeatable, and thus it is suitable for the determination of D-xylose.

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