FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (7): 162-165.doi: 10.7506/spkx1002-6630-201307034

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Rapid Isolation and Identification of β-Lactamase-Producing Strains in Raw Milk

XIE Kun-hao1,2,MAN Chao-xin1,LU Yan1,LIU Shan-shan2,FANG Yu-guo1,YANG Shi-qin2,DONG Xin-yue2,JIANG Yu-jun1,2,*   

  1. 1. National Research Center of Dairy Engineering and Technology, Northeast Agricultural University, Harbin 150030, China; 2. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University,Harbin 150086, China
  • Received:2011-12-23 Revised:2013-01-31 Online:2013-04-15 Published:2013-03-20
  • Contact: JIANG Yu-jun E-mail:yujun_jiang@163.com

Abstract: Penicillin-Micrococcus luteus-TSA medium plates were used to establish a method for the rapid isolation of β-lactamase-producing bacteria in raw milk. This method revealed a more convenient and simpler observation than conventional methods, and could provide highly specific isolation. 16S rRNA gene sequence analysis allowed the assignment of the β-lactamase-producing bacterial isolates obtained in this study. As a result, Escherichia coli was the dominant β-lactamase-producing strain in raw milk.

Key words: β-lactamase, raw milk, isolation, identification

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