FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (10): 107-111.doi: 10.7506/spkx1002-6630-201310023

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Separation and Purification of Phycocyanin by Combined Use of Expanded Bed and Packed Bed

GUO Jing，WANG Feng，CUI Zheng-gang，LIU Li-ming   

  1. 1. School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China；
    2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Received:2012-02-05 Revised:2013-02-22 Online:2013-05-25 Published:2013-05-07

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Abstract:

Expanded bed enrichment coupled with impurity removal with a packed bed was used for the separation and
purification of phycocyanin. Crude phycocyanin extract was adsorbed by a self-made expanded bed (400 mm × 16 mm).
The resulting maximum purity (A620 nm/A280 nm) of phycocyanin was 2.02 compared to 0.23 for the crude extract. It was also
found that the phycocyanin concentration of the crude extract was 0.17 mg/mL and increased to 2.15 mg/mL after this
treatment, with a purity of 1.00 and a recovery of 59.45%. In the second step, a packed bed of XAD7HP macroporous resin
(200 mm × 10 mm) was used to remove impurity proteins from the crudely purified product. The maximum purity of
phycocyanin obtained under optimum conditions was 2.20 with a recovery of 58.81%, and the phycocyanin concentration of
the purfied product was 0.87 mg/mL. The recovery of phycocyanin from the initial crude extract was 34.96%.

Key words: expanded bed, phycocyanin, purification, coupling

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