FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (10): 215-218.doi: 10.7506/spkx1002-6630-201310047

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Simultaneous Determination of Arsenic, Selenium and Antimony in Vigna minima (Roxb.) Ohwi et Ohashi Seeds by Microwave Digestion-Hydride Generation-Atomic Fluorescence Spectrometry

GAO Xiang-yang,HAN Shuai,WANG Ying-ying,QIAO Ming-wu   

  1. 1. Department of Food Science, Zhengzhou Institute of Science and Technology, Zhengzhou 450064, China;
    2. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2012-03-12 Revised:2013-03-20 Online:2013-05-25 Published:2013-05-07
  • Contact: GAO xiang-yang E-mail:ndgaoxy@163.com

Abstract:

A method for simultaneous determination of arsenic, selenium and antimony in Vigna minima (Roxb.) Ohwi et
Ohashi seeds by hydride generation-atomic fluorescence spectrometry (AFS) with microwave digestion was established.
This method was also applied for determination of mung beans. Under optimized conditions, the concentration of arsenic,
selenium and antimony showed a good linear relationship with the fluorescence intensity and the limits of detection
were 0.020, 0.040 ng/mL and 0.050 ng/mL, respectively. The RSDs for 6 parallem determinations of arsenic, selenium and
antimony were 2.2%, 0.97% and 2.6%, respectively. Average recoveries for antimony ranged from 97.19% to 98.72% (n = 6).
The results of comparative experiments proved that Vigna minima (Roxb.) Ohwi et Ohashi seeds contained a much higher
level of selenium, arsenic content in mung beans from Nanyang was significantly lower than in Vigna minima (Roxb.)
Ohwi et Ohashi seeds and mung beans from Zhengzhou, which was richer in antimony than the other two samples. The
described AFS method has been sucessfully applied for simultanenous determination of arsenic, selenium and antimony with
satisfactory results. It is simple, sensitive, accurate, low-cost and efficient and consequently deserves popularization.

Key words: Vigna minima (Roxb.) Ohwi et Ohashi seeds, microwave digestion, hydride generation-atomic fluorescence spectrometry, arsenic, selenium, antimony, simultaneous determination

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