FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (8): 228-231.doi: 10.7506/spkx1002-6630-201308049

Previous Articles     Next Articles

Simultaneous Determination of 18 Food Additives in Foods by High Performance Liquid Chromatography

LIN Hai-dan,ZOU Zhi-fei,QIN Yan,CHEN Yu-fang,LI Xiao-li,WANG Lan,LIU Zhong-yong   

  1. Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China
  • Received:2011-12-18 Revised:2013-03-18 Online:2013-04-25 Published:2013-05-07
  • Contact: LIN Hai-Dan E-mail:linhaidangz@yahoo.com.cn

Abstract: A high performance liquid chromatography method was developed for simultaneous determination of 18 food additives in foods. Low fat or fat free food samples were extracted and purified with n-hexane saturated acetonitrile containing ascorbyl palmitate-6 mol/L HCl solution-saturated sodium chloride solution (containing medium fat sample usingnhexane to remove fat). Oil samples were extracted with n-hexane saturated acetonitrile (containing ascorbyl palmitate)- acetonitrile saturated n-hexane. Analysis was performed using HPLC system equipped with an Ecosil C18 column (250 mm × 4.6 mm, 5 μm) and a UV detector at 280 nm with a mobile phase of acetonitrile-0.6% acetic acid solution, at a flow rate of 1.0 mL/min with gradient elution. Good linear was observed in the range of 1.0–25 mg/L with correlation coefficient of 0.99. The average recovery rate in foods was in the range of 88.9%–99.9% at three spiked levels of 10, 25 mg/kg and 50 mg/kg (n = 6), and the relative standard deviation was in the range of 2.43%–11.7%. The limit of quantification was 10 mg/kg. This method is simple, accurate and suitable for the determination of 18 food additives in foods.

Key words: high performance liquid chromatography (HPLC), foods, food additives

CLC Number: