Abstract: In order to explore a novel method for the rapid determination of neomycin residues in milk, ultra high temperature (UHT)-sterilized pure milk from supermarket was used as the sample to determine neomycin residues in milk on basis of the reaction between neomycin and acidic fuchsin in Britton-Robinson buffer at pH 5.50 through resonance light scattering (RLS) technology. Results indicated that the maximum RLS peak was observed at 625 nm. A good liner relationship (r = 0.9978) was observed in chlorpyrifos concentration range of 0.00–2.50 μg/mL and the detection limit of neomycin was 0.013 μg/mL. The relative standard deviations (RSD) of the precision tests for 5 repeated determinations of all samples were less than 2.5%. The recovery rate of this developed method was in the range of 84.0%–96.7%. Due to simple operation, rapid determination and high sensitivity, this developed method is suitable for the determination of neomycin residues in milk.

Key words: resonance light scattering, neomycin, acidic fuchsin, milk