FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (6): 37-41.doi: 10.7506/spkx1002-6630-201306008

Previous Articles     Next Articles

Optimization of coagulation process of " Qula" rennet casein using response surface methodology

JI Yin-li1,HE Xiao1,GAO Wei-dong1,SONG Li1,GE Jing-wei1,SU Yan-ping1,XIE Xiao-dong2,*   

  1. 1. Gansu Hualing Biotechnical Research Center, Lanzhou 730000, China;2. Lanzhou University, Lanzhou 730070, China
  • Received:2011-12-24 Revised:2013-01-24 Online:2013-03-25 Published:2013-03-01

Abstract: In this study, Qula was selected as the raw material and Pusillus mucor chymosin was used as the coagulant to prepare Qula rennet casein. Based on the single factor tests, response surface design was used to optimize the coagulation process of Qula rennet casein. Results indicated that the order for affecting the yield of Qula rennet casein was curd pH, chymosin addition amount, CaCl2 addition amount, and curd temperature. The optimal curd conditions included pH 6.2, chymosin addition amount of 0.05 g/kg, CaCl2 addition amount of 2.5% and curd temperature of 42 ℃. Under these optimal conditions, the maximum predicted yield was 75.23% compared to 73.54% in the validation experiments.

Key words: Qula, chymosin casein, response surface design, process optimization

CLC Number: