Abstract:

This study aimed at establishing the optimum conditions for purifying total flavonoids from Artemisia selengensis
Turcz with AB-8 macroporous resin. In addition, we preliminarily identified the resulting purified product by means of
chromogenic reaction and UV adsorption spectroscopy. AB-8 possessed excellent adsorption and desorption efficiencies for
total flavonoids. The optimum adsorption and desorption conditions were determined as follows: 2.26 BV of 1.476 mg/mL
sample solution at pH 4.0 was flowed through AB-8 column at a rate of 1 mL/min and then desorbed with 1.5—2.0 BV of
60% ethanol at a flow rate of 1 mL/min. The purity of the purified product obtained under these conditions was 30.26%,
compared to 5.80% for the crude total flavonoids extract. Chromogenic reaction and UV characteristic adsorption indicated
that total flavonoids from Artemisia selengensis Turcz might contain flavonols, isoflavones, flavonone and double hydrogen
flavonols with ortho-dihydroxyl groups in their structure.

Key words: Artemisia selengensis Turcz, total flavonoids, AB-8 macroporous resin, purification, preliminary identification