Comparative Analysis of Fatty Acid and Phospholipids in Viscera of Male and Female Haliotis discus hannai Ino

doi:10.7506/spkx1002-6630-201310040

Abstract

Abstract:

Objective: To describe comparative analysis of visceral fatty acids and phospholipids in male and female Haliotis
discus hannai Ino. Methods: Visceral total lipids were extracted using Folch method and determined for the contents of free
fatty acids, triglycerides, phospholipids and cholesterol. The fatty acid composition was analyzed by GC after derivatization
with 10% H2SO4-CH3OH solution. Phospholipids were extracted using SPE method, and then analyzed by HPLC-ELSD.
Results: The visceral content of unsaturated fatty acids in female Haliotis discus hannai Ino was higher than in male Haliotis
discus hannai Ino. The composition of visceral phospholipids in male and female Haliotis discus hannai Ino, though
complicated, mainly consisted of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS).
Conclusion: The viscera of both female and male Haliotis discus hannai Ino are rich in phospholipids and polysaturated fatty
acids and have high medicinal and edible values.

Key words: viscera of Haliotis discus hannai Ino, fatty acids, phospholipids, gas chromatography (GC), high performance liquid chromatography-evaportive light scattering detector (HPLC-ELSD)

CLC Number:

LIU Yan-qing，LI Zhao-jie*，LI Guo-yun，LIU Xiao-fang，XUE Chang-hu. Comparative Analysis of Fatty Acid and Phospholipids in Viscera of Male and Female Haliotis discus hannai Ino[J]. FOOD SCIENCE, 2013, 34(10): 184-186.

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