Hypolipidemic Effect of Lactobacillus with Bile Salt Hydrolase Activity in Hyperlipidemic Rats

Abstract

Abstract: Objective: To screen Lactobacillus with bile salt hydrolase (BSH) activity and to explore its hypolipidemic effect in hyperlipidemic rats. Methods: Agar plate assay and high-performance liquid chromatography (HPLC) were used to detect and quantify bile salt hydrolase activity in Lactobacillus. Lactobacillus with excellent BSH activity was administrated by gavage to hyperlipidemic rats. Twenty-four male Wistar rats of similar body weight were divided into normal control, hyperlipidemia model and Lactobacillus treatment groups. The latter two groups were fed high fat diet. Three groups were administrated by gavage with 0.9% normal saline, skim milk and skim milk inoculated with Lactobacillus once daily for 21 consecutive days and fasted overnight after the last administration. On the following day, blood and livers were taken for the determination of serum lipid indicators. Besides, rat stools were collected during the last three days of administration and tested for TC, total bile acid (TBA) and short-chain fatty acid (SCFA) levels. Results: Lactobacillus fermentum MGH13-1 showed excellent BSH activity. Serum of TC, TG and LDL-C levels and liver TC level were significantly lower, while fecal TC, TBA and SCFA levels were significantly higher in MGH13-1 treatment group, when compared to high fat model group (P ＜0.05). Conclusion: Lactobacillus MGH13-1 has a remarkable hypolipidemic effect in hyperlipidemic rats.

Key words: Lactobacillus, L. fermentum MGH13-1, bile salt hydrolase, hyperlipidemic rat, hypolipidemic effect

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References

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