FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (10): 187-190.doi: 10.7506/spkx1002-6630-201310041

Previous Articles     Next Articles

Determination of 17α-Methyltestosterone Residue in Water and Sediment from Tilapia Ponds by High Performance Liquid Chromatography

DENG Jian-chao,LI Lai-hao,YANG Xian-qing*,CEN Jian-wei,WU Yan-yan,CHEN Sheng-jun,QI Bo   

  1. Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, National R&D Center for Aquatic Product Processing,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
  • Received:2012-01-19 Revised:2013-04-02 Online:2013-05-25 Published:2013-05-07
  • Contact: Deng Jian-Ming E-mail:djc9801@foxmail.com

Abstract:

A high performance liquid chromatography (HPLC) method has been established for the determination of
17α-methyltestosterone (17α-MeT) in water and sediment of tilapia ponds. 17α-MeT in water and sediment samples was
extracted using methylene chloride and methanol, respectively, followed by rotary evaporation. Then, the residues were
dissolved in methanol. Finally, 17α-MeT was detected using a UV detector and quantified by external standard method. The
average recovery rates of water spiked with 17α-MeT at 1.00–25.0 μg/L were 93.3%–101% with RSD of 3.0%–5.5%. The
average recovery rates of sediment spiked with 17α-MeT at 10–250 μg/kg were 88.7%–96.6% with RSD of 2.7%–6.2%. The
limits of detection for 17α-MeT in water and sediment were 0.5 μg/L and 5.0 μg/kg, respectively. The established method is
sensitive, accurate and suitable for the determination of 17α-MeT residues in aquaculture water and sediment samples.

Key words: 17&alpha, -methyltestosterone;high performance liquid chromatography;aquatic water;sediment

CLC Number: