Optimization of Fermentation Conditions for Sisomicin Production by Marine Streptomyces sp. GB-2

doi:10.7506/spkx1002-6630-201311045

Abstract

Abstract:

The marine Streptomyces sp. GB-2 has been observed for the first time as a new sisomicin-produced strain except
Micromonospora. In this study, Plackett-Burman screening design was undertaken to evaluate the effects of 9 factors. By the
statistical regression analysis, soybean flour, glucose, temperature, and speed were indentified as significant factors affecting
sisomicin fermentation by marine Streptomyces sp. GB-2. In the second phase of the optimization process, the response
surface methodology (RSM) was used to establishe the optimal concentration levels of and the relationships between these
factors. By resolving the quadratic regression model equation using appropriate statistic method, the R2 was estimated as
0.9669 which showed a high correlation. The optimal concentration of the variables were determined as: soybean flour
11.95 g/L, glucose 2.96 g/L, temperature at 28 ℃, and speed 183 r/min. The yield of sisomicin obtained under the optimized
conditions was 726.11 U/mL, an 87.61% increase compared to 387.03 U/mL before the optimization. Accordingly, the
optmized fermentation conditions presented in this study can be considered reliable.

Key words: marine Streptomyces sp. GB-2, Sisomicin, fermentation conditions, optimization

CLC Number:

TS201.3

LI Jin-liang，ZHANG Li，LI Chun-yan，PENG Yi-kai，WANG Ying，Lü Feng-xia，LU Zhao-xin*. Optimization of Fermentation Conditions for Sisomicin Production by Marine Streptomyces sp. GB-2[J]. FOOD SCIENCE, 2013, 34(11): 208-212.

References

[1] EDSON R S, KEYS T F. The Aminoglycosides - Streptomycin, Kanamycin, Gentamicin, Tobramycin, Amikacin, Netilmicin, Sisomicin[J]. Mayo Clinic Proceedings, 1983, 58(2): 99-102.
[2] GUARY D R P. Netilmicin (Netromycin, Schering-Plough)[M]. Drug Intelligence & Clinical Pharmacy, 1983, 17(2): 83-91.
[3] LI B, VAN S A, HOOGMARTENS J, et al. Characterization of impurities in sisomicin and netilmicin by liquid chromatography/mass spectrometry[J]. Rapid Communications in Mass Spectrometry, 2008, 22(22): 3455-3471.
[4] TAWA R, MATSUNAGA H, FUJIMOTO T. High-performance liquid chromatographic analysis of aminoglycoside antibiotics[J]. Journal of Chromatography A, 1998. 812(1-2): 141-150.
[5] 陈剑锋, 陈浩, 张元兴, 等. 西索米星发酵工艺条件的优化[J]. 过程工程学报, 2006, 6(03): 445-449.
[6] 朱坚屏, 倪雍富, 荣洁. 提高西索米星生产菌产量的遗传育种[J]. 中国抗生素杂志, 1998, 23(02): 52-55.
[7] 陈剑锋, 邵敬伟, 张元兴, 等. 西索米星分批发酵过程动力学模型[J]. 福州大学学报(自然科学版), 2007, 35(03): 475-480.
[8] 陈剑锋, 杨志钧, 郭养浩, 等. 有机酸在西索米星发酵过程中的影响[J]. 福州大学学报(自然科学版), 2002, 30(S1): 769-773.
[9] 陈剑锋, 张元兴, 郭养浩, 等. 磷酸盐对西索米星发酵过程的影响作用研究[J]. 中国抗生素杂志, 2002, 27(08): 452-455.
[10] 赵敏, 范瑾, 刘军, 等. 产生西索米星的棘孢小单孢阻断型突变株JIM-121的筛选和研究[J]. 中国抗生素杂志, 1992, 17(01): 16-20.
[11] 范铭琦, 赵敏. 氨基糖苷类抗生素产生菌的代谢调控与菌种选育[J]. 中国抗生素杂志, 2005, 30(09): 68-72.
[12] SZCZEPANIK W, KREZEL A, BRZEZOWSK A M, et al., Impact of Cu(II) ions on the structure and antimicrobial properties of sisomicin, an aminoglycoside antibiotic[J]. Inorganica Chimica Acta, 2008, 361(9-10): 2659-2666.
[13] 洪文荣, 石贤爱, 林娟, 等. 伊尼奥小单孢工程菌S_(96)深层优化培养特性研究[J]. 中国药科大学学报, 2003, 34(05): 83-86.
[14] 刘姝, 陆兆新, 吕凤霞, 等. 一株海洋放线菌的分类鉴定及抗菌活性研究[J]. 南京农业大学学报, 2007, 30(04): 124-129.
[15] 刘姝, 陆颖健, 陆兆新,等. 海洋链霉菌GB-2发酵产物的抗细菌活性及性质研究[J]. 生物工程学报, 2007, 11(06): 1077-1081.
[16] LU Yingjian, DONG Xin, LIU Shu, et al. Characterization and Identification of a Novel Marine Streptomyces sp. Produced Antibacterial Substance[J]. Marine Biotechnology, 2009, 11(6): 717-724.
[17] ZHUANG Yingping, CHEN Bin, CHU Ju, et al. Medium optimization for meilingmycin production by Streptomyces nanchangensis using response surface methodology[J]. Process Biochemistry, 2006, 41(2): 405-409.
[18] MAO Xiangzhao, SHEN Yaling, YANG Liang, et al. Optimizing the medium compositions for accumulation of the novel FR-008/Candicidin derivatives CS101 by a mutant of Streptomyces sp. using statistical experimental methods[J]. Process Biochemistry, 2007, 42(5): 878-883.
[19] MONTGOMERY D C, RUNGER G C, HUBELE N F. Engineering Statistics[M]. John Wiley & Sons Inc., 2008, United States of America.
[20] MU Wanmeng, CHEN Chao, LI Xingfeng, et al., Optimization of culture medium for the production of phenyllactic acid by Lactobacillus sp. SK007[J]. Bioresource Technology, 2009, 100(3): 1366-1370.
[21] ELIBOL, MURAT. Optimization of medium composition for actinorhodin production by Streptomyces coelicolor A3(2) with response surface methodology[J]. Process Biochemistry, 2004. 39(9): 1057-1062.
[22] BEZERRA M A, SANTELLI R E, OLIVEIRA E P, et al. Response surface methodology (RSM) as a tool for optimization in analytical chemistry[J]. Talanta, 2008,76(5), 965-977.
[23] LI Liangzhi, ZHENG Hui and XIAN Mo. Optimization of the medium for streptolydigin production by Streptomyces lydicus AS 4.2501 with precursor feeding using response surface methodology[J]. Journal of the Taiwan Institute of Chemical Engineers, 2010, 41(3): 252-258.
[24] 国家药典委员会. 中华人民共和国药典[M]. 化学工业出版社, 2005(附录Ⅺ): 附录79–84.
[25] SINGH V, TRIPATHI C K M. Production and statistical optimization of a novel olivanic acid by Streptomyces olivaceus MTCC 6820[J]. Process Biochemistry, 2008, 43(11): 1313-1317.
[26] ADINARAYANA K, ELLAIAH P, SRINIVASULU B,et al. Response surface methodological approach to optimize the nutritional parameters for neomycin production by Streptomyces marinensis under solid-state fermentation[J]. Process Biochemistry, 2003. 38(11): 1565-1572.
[27] GU Xiaobo, ZHENG Zongming, YU Haiqing, et al. Optimization of medium constituents for a novel lipopeptide production by Bacillus subtilis MO-01 by a response surface method[J]. Process Biochemistry, 2005, 40(10): 3196-3201.
[28] 胡建恩, 曹茜, 杨帆, 等. 耐高温α-淀粉酶高密度高表达发酵条件的优化[J]. 食品科学, 2012, 33(01): 219-225.
[29] 申乃坤, 王青艳, 陆雁, 等. 响应面法优化耐高温酵母生产高浓度乙醇[J]. 生物工程学报, 2010, 26(01): 42-47.
[30] RAZA W, WU Hongsheng, SHEN Qingrong. Use of response surface methodology to evaluate the effect of metal ions (Ca2+, Ni2+, Mn2+, Cu2+) on production of antifungal compounds by Paenibacillus polymyxa[J]. Bioresource Technology, 2010, 101(6): 1904-1912.
[31] 陈剑锋, 王航, 张元兴, 等. 促甲基化因子对西索米星发酵的影响[J]. 微生物学通报, 2006, 33(04): 85-90.
[32] Gu S B, Li S C, Feng H Y, et al. A novel approach to microbial breeding—low-energy ion implantation[J]. Applied Microbiology and Biotechnology, 2008, 78: 201-209.
[33] SONG He, CHEN Xiaochen, CAO Jiaming, et al. Directed breeding of an Arthrobacter mutant for high-yield production of cyclic adenosine monophosphate by N+ ion implantation[J]. Radiation Physics and Chemistry, 2010, 79(8): 826-830.
[34] 朱坚屏, 倪雍富. 西索米星发酵工艺的优化控制[J]. 生物技术, 2000, 10(06): 25-28.
[35] KUDO F, TAMEGAI H, FUJIWARA T, et al. Molecular cloning of the gene for the key carbocycle-forming enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics and its comparison with dehydroquinate synthase[J]. Journal of Antibiotics, 1999, 52(6): 559-571.
[36] DOUBLET B, WEILL F X, FABRE L, et al. Variant Salmonella genomic island 1 antibiotic resistance gene cluster containing a novel 3 '-N-aminoglycoside acetyltransferase gene cassette, aac(3)-id, in Salmonella enterica serovar Newport[J]. Antimicrobial Agents and Chemotherapy, 2004, 48(10): 3806-3812.