Antioxidant Activity of Eupolyphaga sinensis Peptides

doi:10.7506/spkx1002-6630-201305050

Abstract

Abstract: Objective: To investigate the antioxidant activity of Eupolyphaga sinensis peptides in vivo and in vitro. Methods: The scavenging capacity of Eupolyphaga sinensis peptides against hydroxyl, superoxide anion, and DPPH free radicals in vitro were evaluated. The activities of CAT, GSH-Px and T-SOD, hydroxyl radical scavenging rate and the content of MDA in plasma and liver in D-galactose induced aging model mice were analyzed after feeding hydrolyzed peptides from Eupolyphaga sinensis. Results: Eupolyphaga sinensis peptides had strong scavenging effect on hydroxyl, superoxide anion and DPPH free radicals with IC50 of 0.40, 8.73 mg/mL and 1.32 mg/mL, respectively. Compared to the aging model group, the peptides significantly reduced the content of MDA (P ＜ 0.01), increased the activities of CAT and GSH-Px (P ＜ 0.05, P ＜ 0.01) and hydroxyl radical scavenging rate (P ＜ 0.05) in both plasma and liver, and also enhanced the activity of T-SOD in liver (P ＜ 0.05). Conclusion: Eupolyphaga sinensis peptides have obvious antioxidant activity.

Key words: Eupolyphaga sinensis, peptides, antioxidant activity, free radicals, mice

CLC Number:

TS201.4

PIAO Mei-zi，WANG Mao-cun，WANG Xiao-dong. Antioxidant Activity of Eupolyphaga sinensis Peptides[J]. FOOD SCIENCE, 2013, 34(5): 242-245.

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