Effect of Methyl Jasmonate Treatment on Synthesis and Antioxidant Activity of Phenolic Acids in Grape Berries and Corresponding Mechanisms

doi:10.7506/spkx1002-6630-201306058

Abstract

Abstract: In the present study, the effect of 10 μmol/L MeJA (methyl jasmonate) treatment on the synthesis and antioxidant activity of phenolic acids in grape berries (Vitis vinifera L. × V. labrusca L. cv. ‘Kyoho’) during storage at 1 ℃ was investigated and the corresponding mechanisms were also explored based on phenylpropnaoid metabolism. The results showed that MeJA treatment could significantly inhibit the decay rate and abscission rate of grape berries, induce the increase in activities of key enzymes involved in phenylpropnaoid metabolism including phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H) and chalcone synthase (CHS) and delay the decrease in 4-CL activity. Meanwhile, a remarkable enhancement of phenolic acids (gallic acid, caffeic acid, protocatechuic acid, syringic acid, p-coumaric acid) and total phenolics was observed, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity and reducing power of grape berries during storage was maintained. These results implied that MeJA treatment markedly promoted the synthesis of phenolic acids and maintained antioxidant activity through the regulation of phenylpropanoid-related enzymes, thus finally improving the edible and nutritional quality of postharvest grape berries.

Key words: grape, methyl jasmonate (MeJA), phenolic acids, antioxidant, phenylpropnaoid metabolism

CLC Number:

TS255.3

References

[1] YILMAZ Y, TOLEDO R T. Major flavonoids in grape seeds and skins: antioxidant capacity of catechin, epicatechin, and gallic acid [J]. Journal of Agricultural and Food Chemistry, 2004, 52(2): 255-260
[2] DARIAS-MARTíN J, MARTíN-LUIS B, CARRILLO-LOPEZ M, et al. Effect of caffeic acid on the color of red wine [J]. Journal of Agricultural and Food Chemistry, 2002, 50(7): 2062-2067.
[3] ROBBINS R J. Phenolic acids in foods: an overview of analytical methodology [J]. Journal of Agricultural and Food Chemistry, 2003, 51(10): 2866-2887.
[4] CHEN Jianye, WEN Pengfei, KONG Weifu, et al. Effect of salicylic acid on phenylpropanoids and phenylalanine ammonia-lyase in harvested grape berries [J]. Postharvest Biology and Technology, 2006, 40(1): 64-72.
[5] CREEMAN R A, MULLET J E. Jasmonic acid distribution and action in plants: Regulation during development and responses to biotic and abiotic stress [J]. Proceedings of the National Academy of Sciences of the USA, 1995, 92(10): 4114-4119.
[6] WANG Kaituo, JIN Peng, SHANG Haitao, et al. Effect of methyl jasmonate in combination with ethanol treatment on postharvest decay and antioxidant capacity in Chinese bayberries [J]. Journal of Agricultural and Food Chemistry, 2010, 58(17): 9597-9604.
[7] 汪开拓, 郑永华, 唐双双, 等. 茉莉酸甲酯对草莓果实采后腐烂、苯丙烷类代谢及抗氧化活性的影响 [J]. 食品科技, 2011, 36(8): 40-46.
[8] CAO Shifeng, ZHENG Yonghua, YANG Zhenfeng et al. Effect ofmethyl jasmonate on quality and antioxidant activity of postharvest loquat fruit [J]. Journal of the Science of Food and Agriculture, 2009, 89(12): 2064–2070.
[9] DIXON R A, ACHNINE L, KOTA P, et al. The phenylpropanoid pathway and plant defence-a genomics perspective [J]. Molecular Plant Pathology, 2002, 3(5): 371-390.
[10] THULKE O, CONRATH U. Salicylic acid has a dual role in the activation of defence-related genes in parsley [J]. The Plant Journal, 1998, 14(1): 35-42.
[11] 汪开拓, 郑永华, 饶遵全. 茉莉酸甲酯抑制贮藏葡萄扩展青霉菌试验及机理研究 [J]. 中国果树, 2011, (5): 20-24.
[12] ZUCKER M. Sequential induction of phenylalanineammonia lyase and a lyase-inactivating system in potato tuber disks [J]. Plant Physiology, 1968, 43(3): 365-374.
[13] LAMB C J, RUBERY P H. A spectrophotometric assay for traps-cinnamic acid 4-hydroxylase activity [J]. Analytical Biochemistry, 1975, 68(2): 554-561.
[14] KNOBLOCH K H, HAHLBROCK K. 4-Coumarate: CoA ligase from cell suspension culture of Petroselinum hortense Hoffm. Partial purication, substrate specificity, and further properties [J]. Archives of biochemistry and biophysics, 1977, 184(1): 233-248.
[15] SALEH N A M, FRITSCH H, KREUZALER F, et al. Flavanone synthase from cell suspension cultures of Haplopappus gracilis and comparison with the synthase from parsley [J]. Phytochemistry, 1978, 17(2): 183-186.
[16] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle-dye binding [J]. Analytical Biochemistry, 1976, 72(1): 248-254.
[17] SLINKARD K, SINGLETON V L. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent [J]. Methods in Enzymology, 1999, 299(1): 152-178.
[18] LARRAURI J A, SANCHEZ-MORENO C, SAURA-CALIXTO F. Effect of temperature on the free radical scavenging capacity of extracts from red and white grape pomace peels [J]. Journal of Agricultural and Food Chemistry, 1998, 46(7): 2694-2 697.
[19] OZSOY N, CAN A, YANARDAG R, et al. Antioxidant activity of Smilax excelsa L. leaf extracts [J]. Food Chemistry, 2008, 110(3): 571-583.
[20] HAHLBROCK K, SCHEEL D. Physiology and Molecular Biology of Phenylpropanoid Metabolism [J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1978, 40(1): 347-369.
[21] CHARLES M T, MERCIER J, MAKHLOUF J, et al. Physiological basis of UV-C-induced resistance to Botrytis cinerea in tomato fruit I. Role of pre- and post-challenge accumulation of the phytoalexin-rishitin [J]. Postharvest Biology and Technology, 2008, 47(1): 10-20.
[22] NAFUSSI B, BEN-YEHOSHUA S, RODOV V, et al. Mode of action of hot-water dip in reducing decay of lemon fruit [J]. Journal of Agricultural and Food Chemistry, 2001, 49(1): 107-113.
[23] SANCHEZ-BALLESTA M T, JIMéNEZ J B, ROMERO I, et al. Effect of high CO2 pretreatment on quality, fungal decay and molecular regulation of stilbene phytoalexin biosynthesis in stored table grapes [J]. Postharvest Biology and Technology, 2006, 42(3): 209-216.
[24] SEO H S, SONG J T, CHEONG J J, et al. Jasmonic acid carboxyl methyl transferase: A key enzyme for jasmonate-regulated plant responses [J]. Proceedings of the National Academy of Sciences of the USA, 2001, 98(8): 4788-4793.
[25] BELHADJ A, TELEF N, SAIGNE C, et al. Effect of methyl jasmonate in combination with carbohydrates on gene expression of PR proteins, stilbene and anthocyanin accumulation in grapevine cell cultures [J]. Plant Physiology and Biochemistry, 2008, 46(4): 493-499.
[26] WANG S Y, BOWMAN L, DING M. Methyl jasmonate enhances antioxidant activity and flavonoid content in blackberries (Rubus sp.) and promotes antiproliferation of human cancer cells [J]. Food Chemistry, 2008, 107(3): 1261-1269.