Optimization of Extraction of Pepsin-soluble Collagen from Anglerfish Skin

doi:10.7506/spkx1002-6630-201307045

Abstract

Abstract: Response surface methodology was applied to establish the optimum conditions for extracting pepsin soluble collagen (PSC) from anglerfish skin. Three independent variables including liquid-to-solid ratio, enzyme loading and hydrolysis time were primarily investigated by one-factor-at-a-time design. Further, Box-Behnken optimization design was used to optimize three different levels of these experimental variables. Additionally, this study characterized physiochemical properties of PSC from anglerfish skin. The optimum process conditions for PSC extraction from anglerfish skin were found to be extraction at 4 ℃ for 48 h with an enzyme loading of 3% and a liquid-to-solid ratio of 18:1. Under the optimized conditions, the extraction efficiency of PSC from anglerfish skin was 2.11%. The PSC extract obtained were determined to contain 16% Arg and 18% Tyr. DSC showed a shrinkage temperature of 48.9 ℃ from the PSC extract. SDS-PAGE suggested that the triplehelical structure of collagen basically remained intact. The PSC exhibited a multiporous network structure under SEM.

Key words: collagen, anglerfish, PSC, network structure

CLC Number:

TS254.9

WANG Pei，SU Xiu-rong*，ZHANG Yong-fei，LI Xing-bo，SUN Jing. Optimization of Extraction of Pepsin-soluble Collagen from Anglerfish Skin[J]. FOOD SCIENCE, 2013, 34(7): 217-222.

References

[1] 卓素珍, 张虹, 戴志远. 鮟鱇鱼皮中胶原蛋白的提取及性质研究[J]. 食品科学, 2009, 9(06): 34-40.
[2] 陈小娥, 方旭波, 钟秋琴. 安康鱼皮中胶原蛋白的提取工艺研究[J]. 食品工业科技, 2007, 28(03): 131-133.
[3] 蒋挺大, 张春萍. 胶原蛋白[M]. 北京: 化学工业出版社, 2001: 1-19.
[4] 刘石生, 易美华, 黄文艳. 热水提取罗非鱼鱼鳞胶原蛋白的研究[J]. 现代食品科技, 2006, 22(4): 139-141.
[5] 苏秀榕, 孙蓓, 李妍妍等. 星虫胶原蛋白的生物学特征研究[J]. 天然产物研究与开发, 2009, 21: 48-52.
[6] 丁进峰, 苏秀榕, 张春丹等. 海蜇胶原蛋白提取工艺及促愈合作用研究[J]. 食品科学, 2010, 31(24): 19-23.
[7] 张培丽, 周爱梅, 刘欣等. 淡水鱼皮胶原蛋白提取工艺研究(Ⅰ)[J]. 食品与发酵工业, 2006, 32(12): 150-153.
[8] 郭恒斌, 曾庆祝. 酶法提取鱿鱼皮胶原蛋白工艺条件的研究[J]. 南方水产, 2008, 04(01): 58-63.
[9] 马俪, 王晓丹, 程贺等. 响应面法优化酶法提取鳕鱼皮中胶原蛋白条件[J]. 中国酿造, 2010, 9: 83-85.
[10] Prabjeet Singh, Soottawat Benjakul, Sajid Maqsood, Hideki Kishimur. Isolation and characterisation of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus)[J]. Food Chemistry, 2011, 124: 97-105.
[11] 宁正祥. 食品分析手册[M]. 北京: 中国轻工业出版社, 1998: 263-344.
[12] 陈道才, 励军. 艘鲸鱼中羟脯氨酸的提取与应用[J]. 现代农业科技, 2007, 5: 98-103.
[13] 杨树奇, 曾少葵, 周春霞等. 3种鱼皮的基本成分及氨基酸组成分析[J]. 广东海洋大学学报, 2010, 30(1): 97-100.
[14] 张慧娟, 张晖, 王立, 郭晓娜. 响应面法优化脱脂米糠蛋白提取工艺[J]. 中国油脂, 2009, 34(1): 26-29.
[15] 成海平, 董方霆, 王京兰等. 氨基酸组成分析直接鉴定SDS-PAGE分离的蛋白质[J]. 军事医学科学院院刊, 2001, 25(1): 71-72.
[16] ZHANG Min, LIU Wentao, LI Guoying. Isolation and characterisation of collagens from the skin of largefin longbarbel catfish (Mystus macropterus)[J]. Food Chemistry, 2009, 115: 826-831.
[17] 谢孟峡, 刘媛. 红外光谱酰胺III带用于蛋白质二级结构的测定研究[J]. 高等学校化学学报, 2003, 2(24): 226-231.
[18] Doyle B B, Bendit E G, Blout E R. Infrared spectroscopy of collagen and collagen-like polypeptides[J]. Biopolymers, 1975, 14(5): 937-957.
[19] 苏秀榕, 孙蓓, 李研妍等. 星虫体壁胶原蛋白的提取与特性研究[J]. 天然产物研究与开发, 2009, 01: 51-55.
[20] Plepis A M G, Das-Gupta D K, Goissis G. Pyroelectric properties of anionic collagen and anionic collagen: P(VDE/TRFE)composites[J]. International Symposium on Electrets, 1999, 10: 233-236.
[21] Myung C C, Junzo T. FT-IR study for hydroxyapatie/collagen nanocomposite cross-linked by glutaraldehyde[J]. Biomaterials, 2002, 23: 4811-4818.