FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (10): 31-34.doi: 10.7506/spkx1002-6630-201310007

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Optimization of Ultrasonic Extraction Process for C-S lyase from Lentinus edodes Using Response Surface Methodology

LIU Ying,YUAN Yan,LEI Xiao-yu,HUANG Wen,WANG Yi*   

  1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2012-03-27 Revised:2013-03-22 Online:2013-05-25 Published:2013-05-07
  • Contact: Wang Yi E-mail:lyduandi@live.cn

Abstract:

Response surface methodology was used to optimize the ultrasonic-assisted extraction of C-S lyase from
Lentinus edodes. A regression model describing the yield of C-S lyase with respect to extraction time, ultrasonic power
and material-to-liquid ratio was established. Ultrasonic power had the most significant influence on the yield of C-S lyase
followed by extraction time and material-to-liquid ratio. The optimal extraction condition for C-S lyase from Lentinus
edodes was 100.0 W, 21.5 min and a material-to-liquid ratio of 1:15 (g/mL), and the yield of C-S lyase observed under these
conditions was 48.76 U/g, which was close to the predicted value.

Key words: Lentinus edodes, C-S lyase, ultrasonic, response surface methodology

CLC Number: